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Alternance, année 2020/2021
Laboratoire d’accueil

Plateforme BioPark d’Archamps

Contact administratif/employeur

Dr Philippe BULET (Directeur du BioPark), @ philippe.bulet@biopark-archamps.org, 0450432521 ou 0612777819

Adresse

Plateforme BioPark d’Archamps, Archparc, Bâtiment le Forum 1, 260 Avenue Marie Curie, 74160 Archamps

Titre du Projet de Stage

Étude des effets des stresseurs sur la santé des abeilles en utilisant une approche protéomique

Objectifs

Le sujet de cette alternance portera sur l’évaluation des effets des stresseurs (biotiques et/ou abiotiques) sur la santé de pollinisateurs en utilisant des techniques complémentaires de spectrométrie de masse. Ce travail ‘inscrit dans le cadre du projet européen « PoshBee » initié mi-2018 pour une durée de 5 ans.

Le déclin des pollinisateurs y compris les abeilles présente un problème mondial qui pourrait avoir un lourd impact sur notre environnement et notre économie. Cette baisse serait due aux conditions environnementales dans lesquelles vivent les pollinisateurs. De nombreux chercheurs du monde entier étudient ce sujet et ont pour objectifs de clarifier le rôle et l’impact des facteurs de stress environnementaux (abiotiques et / ou biotiques) sur la santé des pollinisateurs. Néanmoins, d’autres études doivent être menées pour mieux élucider les effets physiologiques des stresseurs sur les pollinisateurs et principalement les perturbations du protéome de l’abeille.

Déroulement de l’alternance

Lors de cette alternance, l’étudiant sera amené à (i) connaitre et préparer le matériel nécessaire pour la collecte du sang d’abeilles (kits de prélèvement à fournir aux différents partenaires européens), (ii) analyser le sang des abeilles en utilisant les techniques de spectrométrie de masse (MALDI et/ou LC-MS/MS), gérer la traçabilité des échantillons de la collecte au stockage post-analyse, et (iv) traiter les données et les résultats au moyen des outils bio-informatiques.

Niveau demandé

Niveau Bac+3

Période de l’alternance

A définir en fonction de la saison apicole

Compétences recherchées

Bonnes bases en biologie et biochimie, compréhension du fonctionnement de la spectrométrie de masse, analyses statistiques, et bon niveau en anglais.

Personne avec qui sera réalisé le travail expérimental d’alternance

Dr Dalel ASKRI

Téléphone : 04 50 43 25 24; Courriel : dalel.askri@biopark-archamps.org

Master 2 internship project, year 2020/2021

Laboratory/Institute: IAB

Director: Pierre HAINAUT

Team (until December 2020): Immunologie Analytique des Pathologies Chroniques

Head of the team: Dr Patrice Marche

Team (from January 2021th): Dept of Signaling Through Chromatin, group RNA and Epigenetics

Head of the team:Dr André Verdel

Name and status of the scientist in charge of the project:Dr Philippe BULET

HDR:  yes

Address:Plateforme BioPark d’Archamps, Bat. Le Forum 1, 260 avenue Marie Curie Archamps Technopole, 74166 Saint Juilen en Genevois Cedex, France

Phone:0450432521e-mail:philippe.bulet@univ-grenoble-alpes.fr

Program of the Master’s degree in Biology:

☑Immunology, Microbiology, Infectious Diseases☐Integrative Structural Biology               

☐Physiology, Epigenetics, Differentiation, Cancer    ☐Neurosciences and Neurobiology       

☐Planta International

Title of the project: Mass spectrometry as innovative tool to investigate the effects of multiple stressors’ exposure on bee health

Objectives (up to 3 lines):

The main objective of this Master 2 internship project will be to investigate the effects of different types of stressors (abiotic and/or biotic) on bee health status using hyphenated mass spectrometry approaches (molecular profiling and proteomics).

Abstract (up to 10 lines):

Pollinator decline presents a real issue that could have a significant impact on environment and economy. Over the last decade, researchers and organisations are dealing with this issue and trying to clarify how the environmental stressors (abiotic and /or biotic) could influence the pollinators. Nevertheless, more extensive investigations need to be conducted to better elucidate the physiological effects of the stressors on pollinators and especially perturbations of the proteomes. We are studying the stressor impact on the haemolymph (insect blood) as the targeted tissue, which may be considered as the mirror of the pollinator health. Proteomics investigations will allow to decipher the altered biological pathways while molecular profiling will allow to have a unique mass fingerprint of the pollinator haemolymph and to elaborate specific models to estimate the stressor impact on bee health. This internship is part of the PoshBee EU project.

Methods (up to 3 lines):

You will have to(i) analyze the haemolymph using MALDI mass fingerprints, (ii) create models using bioinformatics tools, (iii) study the proteome changes by LC-MS/MS and (iv) analyze the biological pathway deregulation using dedicated Bottom-Up proteomics approaches.

Up to 3 relevant publications of the team:

1. Arafah K, Voisin SN, Masson V, Alaux C, Le Conte Y, Bocquet M, Bulet P. MALDI–MS Profiling to Address Honey Bee Health Status under Bacterial Challenge through Computational Modeling. Proteomics 2019, doi: 10.1002/pmic.201900268

2. Masson V, Arafah K, Voisin S, Bulet P. Comparative Proteomics Studies of Insect Cuticle by Tandem Mass Spectrometry: Application of a Novel Proteomics Approach to the Pea Aphid Cuticular Proteins. Proteomics. 2018;18(3-4):10.1002/pmic.201700368. doi:10.1002/pmic.201700368

3. Guschinskaya N, Ressnikoff D, Arafah K, et al. Insect Mouthpart Transcriptome Unveils Extension of Cuticular Protein Repertoire and Complex Organization. iScience. 2020;23(2):100828. doi:10.1016/j.isci.2020.100828

Requested domains of expertise (up to 5 keywords): 

Biology, Mass spectrometry, Chromatography (HPLC), Bioinformatics, Immunology

Language of writing:

French, English

Master 2 internship project , year 2020/2021

Laboratory: Plateforme BioPark d’Archamps

Director: Dr Philippe BULET

Phone: 0450432521 e-mail: philippe.bulet@univ-grenoble-alpes.fr

Address: Plateforme BioPark d’Archamps, Archparc, Bat. Le Forum 1, 260 avenue Marie Curie, 74160 Archamps.

Title of the project: Bioinformatic analyses of bee proteome to investigate the effects of multiple stressors’ exposure on bee health.

Objectives:

The objectives of this Master 2 internship project will be to study the proteome changes on different bee species following exposure to abiotic and/or biotic stressors.

Abstract:

The decline of the pollinator population, and especially bees, is of major concern worldwide. Over the last decade, researchers and organisations are dealing with this issue and trying to clarify how the environmental stressors (abiotic and /or biotic) could influence the pollinator health. Nevertheless, more extensive investigations need to be conducted to better elucidate the molecular perturbations. The bee blood was used as biological matrix. Since several years, we have accumulated proteomics data on the impact of different stressors on different bee species (honeybee, bumble bee and solitary bee) that we are prompting to deeply analyse using appropriate bioinformatic tools. This will allow us to decipher the effect on the bee proteomes and identify the biological pathways deregulated/impacted by different stressors.

Methods:

You will have to (i) analyse the proteome changes using dedicated proteomic tools such as Proteome Discoverer and online proteomic software, (ii) analyse the biological pathways and (iii) perform Gene Ontology analyses to study the biological processes that are deregulated in response to stressors.

Requested domains of expertise:

Biology, Bioinformatics, Statistical analyses

Language of writing:

French, English

Relevant publications of the team:

1. Arafah K, Voisin SN, Masson V, Alaux C, Le Conte Y, Bocquet M, Bulet P. MALDI–MS Profiling to Address Honey Bee Health Status under Bacterial Challenge through Computational Modeling. Proteomics 2019, doi: 10.1002/pmic.201900268

2. Masson V, Arafah K, Voisin S, Bulet P. Comparative Proteomics Studies of Insect Cuticle by Tandem Mass Spectrometry: Application of a Novel Proteomics Approach to the Pea Aphid Cuticular Proteins. Proteomics. 2018;18(3 4): 10.1002/pmic.201700368. doi:10.1002/pmic.201700368

3. Guschinskaya N, Ressnikoff D, Arafah K, et al. Insect Mouthpart Transcriptome Unveils Extension of Cuticular Protein Repertoire and Complex Organization. iScience. 2020;23(2):100828. doi:10.1016/j.isci.2020.100828